Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Decreased CD44v3 expression impairs endometrial stromal cell proliferation and decidualization in women with recurrent implantation failure

doi: 10.1186/s12958-022-01042-w

Figure Lengend Snippet: Demographic characteristics of women recruited in this study

Article Snippet: Human telomerase reverse transcriptase-immortalized human endometrial stromal cells (T-HESCs; which are commonly used to study HESCs and endometrial receptivity) and Ishikawa cells (human endometrial carcinoma cell line, which are commonly used to study endometrial epithelial cells and embryo adhesion), were acquired from the European Collection of Authenticated Cell Cultures (ECACC; Salisbury, UK).

Techniques:

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Decreased CD44v3 expression impairs endometrial stromal cell proliferation and decidualization in women with recurrent implantation failure

doi: 10.1186/s12958-022-01042-w

Figure Lengend Snippet: Expression of CD44v3 in the endometrium of women with RIF ( A ) mRNA levels of CD44s, CD44v3, and CD44v6 in the endometrium of women in the control ( n = 18) and RIF ( n = 24) groups. B Immunohistochemical staining and ( C ) semiquantitative analysis of CD44s, CD44v3, and CD44v6 in the control and RIF groups ( n = 15 per group). D Representative western blots and ( E ) densitometric quantification of CD44v3 in the control and RIF groups. (F) ROC curve for determining the expression of CD44v3 in endometrial tissue. Bar = 50 µm. * p < 0.05; ** p < 0.01

Article Snippet: Human telomerase reverse transcriptase-immortalized human endometrial stromal cells (T-HESCs; which are commonly used to study HESCs and endometrial receptivity) and Ishikawa cells (human endometrial carcinoma cell line, which are commonly used to study endometrial epithelial cells and embryo adhesion), were acquired from the European Collection of Authenticated Cell Cultures (ECACC; Salisbury, UK).

Techniques: Expressing, Control, Immunohistochemical staining, Staining, Western Blot

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Decreased CD44v3 expression impairs endometrial stromal cell proliferation and decidualization in women with recurrent implantation failure

doi: 10.1186/s12958-022-01042-w

Figure Lengend Snippet: Expression of CD44v3 in endometrial tissues and primary endometrial cells after E 2 and P 4 treatments (A)Immunohistochemical staining and (B) semiquantitative analysis of CD44v3 during the proliferative ( n = 12) and mid-secretory phases ( n = 15) in the control patients. (C) Western blot and (D) statistical analyses of CD44v3 expression in the endometrium during the proliferative and mid-secretory phases in the control group. (E and G) Western blot and (F and H) statistical analyses of CD44v3 expression in primary HESCs and HEECs with or without estrogen (E 2 ) and progesterone (P 4 ) treatment for 72 h. P, proliferative phase; MS, mid-secretory phase. Bar = 50 µm. * p < 0.05.

Article Snippet: Human telomerase reverse transcriptase-immortalized human endometrial stromal cells (T-HESCs; which are commonly used to study HESCs and endometrial receptivity) and Ishikawa cells (human endometrial carcinoma cell line, which are commonly used to study endometrial epithelial cells and embryo adhesion), were acquired from the European Collection of Authenticated Cell Cultures (ECACC; Salisbury, UK).

Techniques: Expressing, Immunohistochemical staining, Staining, Control, Western Blot